Asian Journal of Research in Chemistry

Som Shankar Dubey ¹, Battula Sreenivasa Rao ¹, B.V. Kiran ¹

Affiliations
1 Department of Chemistry, GITAM Institute of Technology, GITAM University, Visakhapatnam –530045, Andhra Pradesh

Abstract

Removal of cesium from synthetic aqueous solution through adsorption on illite, under batch equilibrium experimental condition at different values of pH and four temperatures (303, 313, 323, 333 K) was investigated. For this propose the suitability of the Freundlich adsorption model for equilibrium data were investigated. The parameters in the adopted adsorption isotherm models were determined. The study of equilibrium isotherm shows that the best model for analysis of experimental data is freundlich model with correlation coefficient higher than 0.99 (both for temperature and pH). The results showed that increasing of pH and temperature increased the adsorption ability of illite. Optimum conditions for adsorption were determined as T=323 K , pH=9, illite dose=1.5 g and contact time of 24 hr. The negative value of the Gibbs free energy G demonstrates the spontaneous nature of cesium adsorption onto illite

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Battula Sreenivasa Rao ¹, Siva Nagaraju ², B.V. Kiran ³

Affiliations
1 Department of Chemistry, GITAM Institute of Technology, GITAM University, Visakhapatnam, A P, IN
2 Research Scholar, Department of Chemistry, Acharya Nagarjuna University, Guntur A P, IN
3 Research Scholar, Department of Chemistry, GITAM University, Visakhapatnam, A P, IN

Abstract

A simple, selective, rapid, precise and accurate reverse phase high pressure liquid chromatographic method has been developed for simultaneous estimation of Emtricitabine and Tenofovir disoproxil fumerate in pharmaceutical Tablet dosage form. The mobile phase consisted of 65:35 % (v/v) of Methanol and 0.1M of potassium di hydrogen ortho phosphate and pH adjusted to 3.2 with ortho phosphoric acid.The method developed is operated on isocratic mode.The flow rate is 1.0 ml/min. Chromatographic determination of Emtricitabine and Tenofovir disoproxil fumerate was performed on Phenomenex C18 column (150 X 4.6 mm Id, ODS-2, 5μm). The wavelength of detection is 260 nm. The injection volume is 20μL. The retention time of Emtricitabine is 1.92 ± 0.01 minutes while the retention time of Tenofovir disoproxil fumerate is 3.17 ± 0.01minures.The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation, solution stability, ruggedness, and robustness. The influence of Acid, Alkaline, Oxidative Stress, Photolytic stress conditions on Emtricitabine and Tenofovir disoproxil fumerate was studied. Results indicated that Emtricitabine and Tenofovir disoproxil fumerate is stable under the experimental conditions. The proposed method has been successfully used for the estimation in tablet dosage forms.

Keywords

Emtricitabine, Tenofovir Disoproxil Fumerate, Anti-retrovirals, Assay, HPLC

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